Pla2g4a Knockout cell line (Raw 264.7)

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location： Home > Products > KO Cell Lines > Pla2g4a Knockout cell line (Raw 264.7)

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Pla2g4a Knockout cell line (Raw 264.7) Order

Pla2g4a Knockout cell line (Raw 264.7)
Catalog#
YKO-MT0038
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Pla2g4a Knockout cell line (Raw 264.7)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
Pla2g4a Knockout cell line (Raw 264.7)
Species
Mouse
Gene Name
Pla2g4a
Gene ID
18783
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 4 (based on transcript Pla2g4a-201)
Validation: PCR+Sanger Sequencing
Pla2g4a Knockout cell line (Raw 264.7) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Pla2g4a gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Raw 264.7 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Pla2g4a gene knockout Pla2g4a Knockout cell line (Raw 264.7) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
Pla2g4a Knockout cell line (Raw 264.7)
Pla2g4a Knockout cell line (Raw 264.7)
Catalog#
YKO-MT0038
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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    Organism: Human
    Gene Name: PLA2G4A
    Gene ID: 5321
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    Catalog#: YKO-HT0216
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    Gene Name: PLA2G4A
    Gene ID: 5321
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    Catalog#: YKO-H713
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      inquiry   More details

    Organism: Human
    Gene Name: PLA2G4A
    Gene ID: 5321
    Size: 1*10^6
    Catalog#: YKO-HS3910
    YKO-HS3910(HeLa)

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  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • PLA2G4A Knockout cell line (THP-1)

    PLA2G4A Knockout cell line (THP-1)

    Organism: Human
    Gene Name: PLA2G4A
    Gene ID: 5321
    Size: 1*10^6
    Catalog#: YKO-HT0216
      inquiry   More details
  • PLA2G4A Knockout cell line (A549)

    PLA2G4A Knockout cell line (A549)

    Organism: Human
    Gene Name: PLA2G4A
    Gene ID: 5321
    Size: 1*10^6
    Catalog#: YKO-H713
      inquiry   More details
  • PLA2G4A Knockout cell line (HeLa)

    PLA2G4A Knockout cell line (HeLa)

    Organism: Human
    Gene Name: PLA2G4A
    Gene ID: 5321
    Size: 1*10^6
    Catalog#: YKO-HS3910
      inquiry   More details

Pla2g4a Knockout cell line (Raw 264.7) Order

Pla2g4a Knockout cell line (Raw 264.7)
Catalog#
YKO-MT0038
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Pla2g4a Knockout cell line (Raw 264.7)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
Pla2g4a Knockout cell line (Raw 264.7)
Species
Mouse
Gene Name
Pla2g4a
Gene ID
18783
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 4 (based on transcript Pla2g4a-201)
Validation: PCR+Sanger Sequencing
Pla2g4a Knockout cell line (Raw 264.7) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Pla2g4a gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Raw 264.7 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Pla2g4a gene knockout Pla2g4a Knockout cell line (Raw 264.7) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
Pla2g4a Knockout cell line (Raw 264.7)
Pla2g4a Knockout cell line (Raw 264.7)
Catalog#
YKO-MT0038
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • PLA2G4A Knockout cell line (THP-1)

    PLA2G4A Knockout cell line (THP-1)
      inquiry   More details

    Organism: Human
    Gene Name: PLA2G4A
    Gene ID: 5321
    Size: 1*10^6
    Catalog#: YKO-HT0216
    YKO-HT0216(THP-1)
  • PLA2G4A Knockout cell line (A549)

    PLA2G4A Knockout cell line (A549)
      inquiry   More details

    Organism: Human
    Gene Name: PLA2G4A
    Gene ID: 5321
    Size: 1*10^6
    Catalog#: YKO-H713
    YKO-H713(A549)
  • PLA2G4A Knockout cell line (HeLa)

    PLA2G4A Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: PLA2G4A
    Gene ID: 5321
    Size: 1*10^6
    Catalog#: YKO-HS3910
    YKO-HS3910(HeLa)

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Wild-type Cell Lines

  • RAW 264.7 cell line

    Mouse Macrophages transformed by leukemia virus(RAW 264.7)

    Price (USD): -
    Catalog#: YC-C020
    Size: 1*10^6
    Instruction: Mouse Macrophages transformed by leukemia virus(RAW 264.7)
      inquiry   More details

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • PLA2G4A Knockout cell line (THP-1)

    PLA2G4A Knockout cell line (THP-1)

    Organism: Human
    Gene Name: PLA2G4A
    Gene ID: 5321
    Size: 1*10^6
    Catalog#: YKO-HT0216
      inquiry   More details
  • PLA2G4A Knockout cell line (A549)

    PLA2G4A Knockout cell line (A549)

    Organism: Human
    Gene Name: PLA2G4A
    Gene ID: 5321
    Size: 1*10^6
    Catalog#: YKO-H713
      inquiry   More details
  • PLA2G4A Knockout cell line (HeLa)

    PLA2G4A Knockout cell line (HeLa)

    Organism: Human
    Gene Name: PLA2G4A
    Gene ID: 5321
    Size: 1*10^6
    Catalog#: YKO-HS3910
      inquiry   More details

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